Osteoclast Response to Bioactive Surface Modification of Hydroxyapatite
نویسندگان
چکیده
The aim of this study was to evaluate the in vitro response of osteoclast-like cells (RAW 264.7 cells) to a bioactive hydroxyapatite (HAP) surface that was modified using the 30% phosphoric acid-etching procedure reported in our previous paper (2013). The cells on the bioactive HAP surface were multinucleated and were larger than those on the untreated HAP surface. The cell occupancies were greater on the bioactive HAP surface than on the untreated HAP surface at 7, 14, 21, and 28 days of differentiation; in particular, the values at 21 and 28 days were significantly larger (P < 0.05 and P < 0.01, respectively). These findings show that the bioactive HAP surface may enhance the adhesion and differentiation of RAW 264.7 cells as well as osteoblast-like cells, indicating its potential as a superior surface for bone tissue engineering.
منابع مشابه
Fabrication, Characterization and Osteoblast Response of Cobalt-Based Alloy/Nano Bioactive Glass Composites
IIn this work, cobalt-based alloy/ nano bioactive glass (NBG) composites with 10, 15 and 20 wt% NBG were prepared and their bioactivity after immersion in simulated body fluid (SBF) for 1 to 4 weeks was studied. The scanning electron microscopy images of two- step sintered composites revealed a relatively dense microstructure the density of which decreased with the increase in the NBG amount. M...
متن کاملSurface modification for titanium implants by hydroxyapatite nanocomposite
Background: Titanium (Ti) implants are commonly coated with hydroxyapatite (HA). However, HA has some disadvantages such as brittleness, low tensile strength and fracture toughness. It is desirable to combine the excellent mechanical properties of ZrO2 and the chemical inertness of Al2O3 with respect to the purpose of this project which was coating Ti implants with HA-ZrO2-Al2O3 to modify the s...
متن کاملHeat Treatment Of Cobalt-Base Alloy Surgical Implants With Hydroxyapatite-Bioglass For Surface Bioactivation
ASTM F-75 Cobalt-base alloy castings are widely used for manufacturing orthopedic implants. This alloy needs both homogenization and solutionizing heat treatment after casting, as well as bioactivation of the surface to increase the ability of tissue bonding. In this study, ASTM F-75 Cobalt-base substrate was heat treated at 1220°C for 1 hour in contact with Hydroxyapatite-Bioglass powder in or...
متن کاملLaser surface modification of hydroxyapatite and glass-reinforced hydroxyapatite.
Surface treatment of materials with excimer laser radiation often results in the formation of a rough columnar or cone-shaped surface topography, which leads to a considerable increase in the surface area. As a result, the search for a non-porous bioactive material with adequate mechanical properties and a high surface to volume ratio, similar to porous materials, which could be used for drug d...
متن کاملSynthesis of nano HA/βTCP mesoporous particles using a simple modification in granulation method in PVA/ Oil and investigation of biological response
Granulation method in the mixture of oil, water, and PVA was used to synthesize HA/βTCP porous nano-powders. Results showed that the presence of oil in PVA/oil system results in crystallization of mesoporous HA/βTCP particles with the surface area of 93 cm2/g. Using Rietveld method, the amounts of HA/βTCP and amorphous phases in the oven-dried PVA substrate powders after heat-treatment were cal...
متن کامل